We have previously described a virulence gene in Vibrio cholerae (irgA) that is more than 850-fold regulated in response to iron. Negative regulation of irgA by iron occurred at the transcriptional level, and there was a dyad symmetric nucleotide sequence in the vicinity of the irgA promoter homologous to the Fur binding site in Escherichia coli. When irgA was cloned into E. coli, we showed tha...

9 Half-hour latent heat flux (LE) was measured over an early-maturing peach orchard 10 (Prunus persica L.) by two different hygrometers: 1) infrared gas analyzer (IRGA) (Li11 COR, model Li-7500); and 2) ultraviolet hygrometer (Campbell, KH20). A good 12 agreement between LE obtained with the IRGA (LEIRGA) and the KH20 (LEKH20) 13 hygrometers was observed. During rainy periods, LEIRGA and LEKH20...

A common method to estimate the carbon isotopic composition of soil-respired air is to use Keeling plots (delta(13)C versus 1/CO2 concentration). This approach requires the precise determination of both CO2 concentration ([CO2]), usually measured with an infrared gas analyser (IRGA) in the field, and the analysis of delta(13)C by isotope ratio mass spectrometry (IRMS) in the laboratory. We meas...

The Vibrio cholerae fur gene was previously cloned and sequenced. A putative Fur box was identified in the divergent promoters of irgA, a virulence factor of V. cholerae, and irgB, a transcriptional activator of irgA. In this work, V. cholerae Fur was overexpressed in Escherichia coli and purified to approximately 95% homogeneity. The purified protein bound a DNA fragment containing the irgA-ir...

gas analysis (IRGA) and found them to be highly correlated, with r 2 =0.95. The use of IRGA provides the potential for automation in a system due to the electronic output. Bowling et al. (2001) described a system using a pneumatically driven piston to inject sample air, which gave consistency and high accuracy (a coefficient of variance of 0.05%). This and similar systems afford accurate measur...

The concepts of robust classification and intelligently controlling the search process of genetic algorithm (GA) are introduced and integrated with a conventional genetic classifier for development of a new version of it, which is called Intelligent and Robust GA-classifier (IRGA-classifier). It can efficiently approximate the decision hyperplanes in the feature space. It is shown experime...

A hydroponic experiment was carried out to investigate the effect of phosphorus (P) nutrition on arsenic (As) uptake and translocation within the seedlings of rice cultivars. The experiment occurred in three stages: I 5 days of acclimatization (nutritive solution); II 10 days under P (0.0 and 0.09 mM) and As (0.0 and 100 mM) treatments; III 5 days under recovery. The As exposure had significant...

قدرت تفکیک دستگاه طیف‌نگار جرمی به صورت مستقیم شدت یون خروجی و انرژی آن بستگی دارد. مقدار تولید شده جریان باریکه الکترونی وابسته است. از جمله وظایف مدار کنترل چشمه برخورد تنظیم برای رسیدن بهینه می‌باشد. در ساخت پارامترهایی نظیر تثبیت آن، دقیق پتانسل الکتریکی لنز الکترودهای یونی بسیار حایز اهمیت این مقاله با قابلیت انتشار الکترون، پتانسیل نقاط طراحی ساخته شد. کنترلر بر روی طیف نگار 44Varian MAT ...